H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport

Kudo, Michiko; Katayoshi, Takeshi; Kobayashi-Nakamura, Kumiko; Akagawa, Mitsugu; Tsuji-Naito, Kentaro

doi:10.1016/J.BBRC.2016.05.093

Title: H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport

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Abstract

Peptide transporter 2 (PEPT2) is a member of the proton-coupled oligopeptide transporter family, which mediates the cellular uptake of oligopeptides and peptide-like drugs. Although PEPT2 is expressed in many tissues, its expression in epidermal keratinocytes remains unclear. We investigated PEPT2 expression profile and functional activity in keratinocytes. We confirmed PEPT2 mRNA expression in three keratinocyte lines (normal human epidermal keratinocytes (NHEKs), immortalized keratinocytes, and malignant keratinocytes) by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR. In contrast to PEPT1, PEPT2 expression in the three keratinocytes was similar or higher than that in HepG2 cells, used as PEPT2-positive cells. Immunolocalization analysis using human skin showed epidermal PEPT2 localization. We studied keratinocyte transport function by measuring the oligopeptide content using liquid chromatography/tandem mass spectrometry. Glycylsarcosine uptake in NHEKs was pH-dependent, suggesting that keratinocytes could absorb small peptides in the presence of an inward H{sup +} gradient. We also performed a skin-permeability test of several oligopeptides using skin substitute, suggesting that di- and tripeptides pass actively through the epidermis. In conclusion, PEPT2 is expressed in keratinocytes and involved in skin oligopeptide uptake. -- Highlights: •PEPT2 is expressed in keratinocytes, which are more common than other skin cells. •Immunolocalization analysis using human skinmore »« less

Authors:

Kudo, Michiko; Katayoshi, Takeshi; Kobayashi-Nakamura, Kumiko ^[1]; Akagawa, Mitsugu ^[2]; Tsuji-Naito, Kentaro ^[1]

DHC Corporation Laboratories, Division 2, 2-42 Hamada, Mihama-ku, Chiba 261-0025 (Japan)
Department of Biological Chemistry, Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai 599-8531 (Japan)

Publication Date:: Fri Jul 08 00:00:00 EDT 2016

OSTI Identifier:: 22598779

Resource Type:: Journal Article

Journal Name:: Biochemical and Biophysical Research Communications

Additional Journal Information:: Journal Volume: 475; Journal Issue: 4; Other Information: Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA); Journal ID: ISSN 0006-291X

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; COLLAGEN; EPIDERMIS; LIQUID COLUMN CHROMATOGRAPHY; MASS SPECTROSCOPY; MESSENGER-RNA; PEPTIDES; PERMEABILITY; PH VALUE; POLYMERASE CHAIN REACTION; TRANSCRIPTION; UPTAKE

Citation Formats


                    Kudo, Michiko, Katayoshi, Takeshi, Kobayashi-Nakamura, Kumiko, Akagawa, Mitsugu, and Tsuji-Naito, Kentaro. H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport.  United States: N. p., 2016. 
        Web.  doi:10.1016/J.BBRC.2016.05.093.

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                    Kudo, Michiko, Katayoshi, Takeshi, Kobayashi-Nakamura, Kumiko, Akagawa, Mitsugu, & Tsuji-Naito, Kentaro. H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport.  United States.  https://doi.org/10.1016/J.BBRC.2016.05.093

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                    Kudo, Michiko, Katayoshi, Takeshi, Kobayashi-Nakamura, Kumiko, Akagawa, Mitsugu, and Tsuji-Naito, Kentaro. 2016.  
        "H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport".  United States.  https://doi.org/10.1016/J.BBRC.2016.05.093.

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@article{osti_22598779,

  title        = {H{sup +}/peptide transporter (PEPT2) is expressed in human epidermal keratinocytes and is involved in skin oligopeptide transport},

  author       = {Kudo, Michiko and Katayoshi, Takeshi and Kobayashi-Nakamura, Kumiko and Akagawa, Mitsugu and Tsuji-Naito, Kentaro},

  abstractNote = {Peptide transporter 2 (PEPT2) is a member of the proton-coupled oligopeptide transporter family, which mediates the cellular uptake of oligopeptides and peptide-like drugs. Although PEPT2 is expressed in many tissues, its expression in epidermal keratinocytes remains unclear. We investigated PEPT2 expression profile and functional activity in keratinocytes. We confirmed PEPT2 mRNA expression in three keratinocyte lines (normal human epidermal keratinocytes (NHEKs), immortalized keratinocytes, and malignant keratinocytes) by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real-time RT-PCR. In contrast to PEPT1, PEPT2 expression in the three keratinocytes was similar or higher than that in HepG2 cells, used as PEPT2-positive cells. Immunolocalization analysis using human skin showed epidermal PEPT2 localization. We studied keratinocyte transport function by measuring the oligopeptide content using liquid chromatography/tandem mass spectrometry. Glycylsarcosine uptake in NHEKs was pH-dependent, suggesting that keratinocytes could absorb small peptides in the presence of an inward H{sup +} gradient. We also performed a skin-permeability test of several oligopeptides using skin substitute, suggesting that di- and tripeptides pass actively through the epidermis. In conclusion, PEPT2 is expressed in keratinocytes and involved in skin oligopeptide uptake. -- Highlights: •PEPT2 is expressed in keratinocytes, which are more common than other skin cells. •Immunolocalization analysis using human skin revealed epidermal PEPT2 localization. •Keratinocytes could absorb small peptides in the presence of an inward H{sup +} gradient. •Di- and tripeptide pass actively through the epidermis.},

  doi          = {10.1016/J.BBRC.2016.05.093},

  url          = {https://www.osti.gov/biblio/22598779},
  journal      = {Biochemical and Biophysical Research Communications},

  issn         = {0006-291X},

  number       = 4,

  volume       = 475,

  place        = {United States},

  year         = {Fri Jul 08 00:00:00 EDT 2016},

  month        = {Fri Jul 08 00:00:00 EDT 2016}

}

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